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A catalyst-free and environmentally friendly process was employed for the production of biodiesel from sea mango seed oil. This oil is non-edible and contains several fatty acids such as palmitic acid (C16), trans-9-elaidic acid (C18:1t), oleic acid (C18:1), linoleic acid (C18:2), and linolelaidic acid (C18:2n6t). The in situ extraction and transesterification of the oil were carried at the subcritical methanol–water condition. The effect of reaction temperature and time on the yield of biodiesel was studied. The maximum yield of biodiesel was 98.58% and achieved at 200 °C, 40 bar and reaction time of 6 h. The ratio among sea mango seed, methanol, and water was 2:10:1.

Production of biodiesel from sea mango (Cerbera odollam) seed using in situ subcritical methanol–water under a non-catalytic process