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High expression of long intergenic non-protein coding RNA 987 ( LINC00987 ) is strongly associated with low overall survival of osteosarcoma; however, its role in osteosarcoma remains unclear. This study explored the biological function and underlying mechanism of  LINC00987  in osteosarcoma. In this study,  LINC00987  expression in osteosarcoma cells was analyzed using Cancer Cell Line Encyclopedia and qRT-PCR. The proliferation and migration and invasion in osteosarcoma cells were evaluated using Cell Counting Kit-8 and Transwell assays, respectively. Bioinformatic analysis was used to predict the  LINC00987 -bound miRNAs and miR-376a-5p-bound mRNAs. Dual-luciferase reporter assays were used to assess the interaction between miR-376a-5p,  LINC00987 , and forming-binding protein 1 (FNBP1). FNBP1 expression was measured by western blotting.  LINC00987  was found to be upregulated in osteosarcoma cells.  LINC00987  silencing suppressed proliferation, migration, and invasion of osteosarcoma cells. Additionally, miR-376a-5p expression was downregulated in osteosarcoma cells. miR-376a-5p knockdown reversed the effect of  LINC00987  silencing on the biological function of osteosarcoma cell. miR-376a-5p was found to target  LINC00987  and FNBP1. FNBP1protein level was increased in osteosarcoma cells; however, it was inhibited by silencing  LINC00987  and enhanced by silencing miR-376a-5p. In conclusions, this study suggests  LINC00987  silencing inhibits osteosarcoma cell proliferation, migration, and invasion by sponging miR-376a-5p to regulate FNBP1 expression.  LINC00987  as a potential therapeutic target for osteosarcoma.

discover oncology

Silencing long intergenic non-protein coding RNA 00987 inhibits proliferation, migration, and invasion of osteosarcoma cells by sponging miR-376a-5p to regulate FNBP1 expression