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Livestock farming has been an integral part of Nepalese agriculture systems since time immemorial. Giardia duodenalis is a cosmopolitan intestinal parasite present in a wide range of hosts. Very little or no information is reported on the prevalence of giardiasis in livestock of Nepal. This study was done during Jan 18 to July 19, 2014 to determine the prevalence and associated risk factors of Giardia duodenalis infection in dairy cattle of Chitwan, Nepal. A total of 96 fresh fecal samples were collected from various dairy pocket areas and were transferred to collection bottles with 10% formalin. Wet smears of the samples were prepared, stained with lugol's iodine and then viewed under microscope at 400× magnification. The overall prevalence was found to be 44.79% (43/96). Based on the risk factors assessment survey, age was found to be significantly associated with the prevalence of Giardia duodenalis . The prevalence was found significantly ( P  &lt; 0.05) higher in 1-6 months age group compared to &gt; 3 years. Similarly, higher prevalence was found in diarrheic animals compared to their counterparts ( P  &lt; 0.05). Though higher prevalence was recorded in unhygienically housed animals, it was statistically non-significant ( P  &lt; 0.05). Giardiasis should be considered as an important cause of diarrhea and further advanced diagnostic approaches should be employed for the confirmation of giardiasis in dairy cattle.

Prevalence and risk factors associated with Giardia duodenalis infection in dairy cattle of Chitwan, Nepal