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Evaluations were made to assess the efficacy of assembly pheromone, attractant sex pheromone and the kairomone carbon dioxide in attracting the questing larval nymphal and adult stages of the brown ear dog tick, Rhipicephalus sanguineus. Ticks attracted to one location were killed using the acaricide deltamethrin. Initially, simulated field trials were conducted to observe the percentage of attraction of ticks from different distances. Tick lure comprising of carbon dioxide as kairomone and 2,6-dichlorophenol in combination with assembly pheromone and deltamethrin on a thermocol platform was prepared and field trials were conducted in the kennels of Blue Cross Society of India, Velachery, Chennai. The tick lure attracted larval, nymphal and adult stages of R. sanguineus ticks in and around the kennels. Observations were made after 24 h of placing the tick lure. In simulated field out of the 50 ticks used for each distance trials, positive response was exhibited by a total number of 20 (40 %), 17 (34 %) and 14 (28 %) ticks from 0.5, 1 and 1.5 m, respectively. Field trials conducted revealed considerable attraction of larvae and nymph to the tick lure. Few adult ticks (2-5) were also lured to the pheromone impregnated filter paper disc.

Field trials to attract questing stages of brown dog tick, Rhipicephalus sanguineus using tick pheromone–acaricide complex