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Hibiscus moscheutos  L., also known as hardy hibiscus, is valued for its medicinal and ornamental attributes. It is usually propagated via seeds or cuttings. The purpose of this investigation was to develop a dependable micropropagation for  H. moscheutos  ‘Luna White’. To that end, the effect of four explant types (leaf, root, node, shoot tip) and two growth regulators 6-benzylaminopurine (BA) and  meta -Topolin ( mT ) (6-(3-hydroxybenzylamino) purine) on  in vitro  growth of  H. moscheutos  was investigated. Genetic stability of the  in vitro  grown plants was assessed using flow cytometry, and chromosome count was investigated. No shoots were obtained from leaf or root explants. An efficient protocol for micropropagation of  H. moscheutos  using two explant types, 2-node and shoot tip explants, and two cytokinins (BA and  m T) capable of producing true-to-type regenerants was established. Both BA and  mT  can be used at 2 μM or 4 μM using either 2-node or shoot tip explants. No significant difference was found between the nuclear DNA contents of seed-derived and in vitro grown plants ( P  &lt; 0.05). The mean 2C DNA and monoploid 1Cx-values of seed-derived plants were 3.25 ± 0.08 pg and 1.62 ± 0.04 pg, respectively, compared with 3.26 ± 0.06 pg and 1.63 ± 0.02 pg, respectively, for  in vitro  grown plants. The chromosome number of both seed-derived plants and regenerants was determined to be 2 n  = 2 x  = 38. The mature regenerants obtained were fertile and phenotypically similar to seed-derived plants.

in vitro cellular and developmental biology. plant

Micropropagation of Hibiscus moscheutos L. ‘Luna White’: effect of growth regulators and explants on nuclear DNA content and ploidy stability of regenerants