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Late blight is a disease with the biggest economic impact on potato cultivation worldwide. Pyramiding of the resistance genes originating from potato wild relatives is a breeding strategy that has a potential to produce potato cultivars durably resistant to late blight. Growing such cultivars would allow limiting the intensive chemical control of the disease. The goal of this work was to transfer the late blight resistance gene  Rpi-rzc1  from  Solanum ruiz-ceballosii  to the tetraploid level of cultivated potato and to pyramid it with the  Rpi-phu1  gene .  We obtained two diploid and, through 4x-2x cross, a tetraploid potato population segregating for the  Rpi-rzc1  presence, as well as one diploid and one tetraploid population where both genes were introgressed. In total, 754 progeny clones were tested for resistance to late blight in detached leaflet assays. Pathogen isolates avirulent on plants with both genes and virulent on plants with the  Rpi-phu1  were used. The selection was assisted by two PCR markers flanking the  Rpi-rzc1  gene and a newly designed, highly specific intragenic marker indicating the  Rpi-phu1  gene presence. We obtained 26 diploid and 49 tetraploid potato clones with pyramid of both genes that should enhance the durability and spectrum of their late blight resistance and that can be exploited in potato breeding. The specificity of the marker for the  Rpi-phu1  gene and the precision of the  Rpi-rzc1  mapping were improved in this work.

Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di- and tetraploid levels