English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Tomato yellow leaf curl virus (TYLCV) hampers tomato production worldwide. Our previous studies have focussed on mapping and ultimately cloning of the TYLCV resistance genes  Ty - 1  and  Ty - 3 . Both genes are derived from  Solanum chilense  and were shown to be allelic. They code for an RNA-dependent RNA polymerase (RDR) belonging to the RDRγ type defined by a DFDGD catalytic domain. In this study, we first fine-mapped the TYLCV resistance in  S. chilense  LA1932, LA1960 and LA1971. Results showed that chromosomal intervals of the causal genes in these TYLCV-resistant accessions overlap and cover the region where  Ty - 1/Ty - 3  is located. Further, virus-induced gene silencing was used to silence  Ty - 1 / Ty - 3  in tomato lines carrying TYLCV resistance introgressed from  S. chilense  LA1932, LA1938 and LA1971. Results showed that silencing  Ty - 1/Ty - 3  compromised the resistance in lines derived from  S. chilense  LA1932 and LA1938. The LA1971-derived material remained resistant upon silencing  Ty - 1/Ty - 3 . Further, we studied the allelic variation of the  Ty - 1/Ty - 3  gene by examining cDNA sequences from nine  S. chilense -derived lines/accessions and more than 80 tomato cultivars, landraces and accessions of related wild species. The DFDGD catalytic domain of the  Ty - 1/Ty - 3  gene is conserved among all tomato lines and species analysed. In addition, the 12 base pair insertion at the 5-prime part of the  Ty - 1/Ty - 3  gene was found not to be specific for the TYLCV resistance allele. However, compared with the susceptible  ty - 1  allele, the  Ty - 1/Ty - 3  allele is characterized by three specific amino acids shared by seven TYLCV-resistant  S. chilense  accessions or derived lines. Thus,  Ty - 1/Ty - 3 -specific markers can be developed based on these polymorphisms. Elevated transcript levels were observed for all tested  S. chilense  RDR  alleles (both  Ty - 1  and  ty - 1  alleles), demonstrating that elevated expression level is not a good selection criterion for a functional  Ty - 1/Ty - 3  allele.   Electronic supplementary material  The online version of this article (doi:10.1007/s11032-015-0329-y) contains supplementary material, which is available to authorized users.

Assessing the genetic variation of Ty-1 and Ty-3 alleles conferring resistance to tomato yellow leaf curl virus in a broad tomato germplasm