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We demonstrate a strategy for construction of high-throughput microfluidic systems generating gradations of chemistry in micro-droplets. The productivity of the systems that we propose is limited only by the maximum rate of the droplet formation, and does not need to be limited by the rate of mixing. Multilayer polycarbonate chips transform two miscible input streams A and B into N streams of droplets, containing mixtures [A] i , [B] i . Exemplary devices generate linear ([B] i  ∝ i) and logarithmic gradations (ln[B] i  ∝ i). We also analyze the use of the same strategy for the generation of concentration gradation in the streams of droplets comprising mixtures of liquids of different viscosities. The devices preserve the required distribution of compositions, while allowing the volume of the droplets to be tuned over almost two orders of magnitude (i.e. between 3 and 80 nL).

Microfluidic architectures for efficient generation of chemistry gradations in droplets