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The goal of this work was to present two high-performance liquid chromatography (HPLC) method that could be applied for the determination of the total radioactive purity of 2-deoxy-2-[ 18 F]fluoro-D-glucose ([ 18 F]FDG) and  O -(2-[ 18 F]fluoroethyl)-L-tyrosine ([ 18 F]FET). The separation of [ 18 F]fluoride ions, [ 18 F]FET and [ 18 F]FET intermediate was accomplished on LiChrosper RP - 18, 250 × 4 mm, 5 µm (Merck) analytical column. For mobile phase 10 mM potassium dihydrogen phosphate buffer at pH7 (A) and acetonitrile (B) was used: 0–2 min: 15% B; 2–12 min: 85% B; 12–15 min: 15% B, respectively. Analysis of [ 18 F]FDG was performed using LiChrosper 100 NH 2 , 250 × 4.5 mm, 5 µm (Merck) analytical column. The initial mobile phase composition was 10 mM KH 2 PO 4  buffer (pH7) and acetonitrile (15:85, v/v) and the acetonitrile ratio was decreased to 15% at 2 min after the sample injection and held for 5 min. Complete elution of [ 18 F]fluoride ions from stationary phases could be achieved by adding 10 mg/mL K[ 19 F]F to radioactive samples in a ratio 1:1 during the sample preparation. Recovery of [ 18 F]fluoride ions ranged from 99.5 to 100.6%. The validation of the developed methods showed good results for linearity ( r  2  = 0.9981–0.9996), specificity ( R  S  = 3.7–10.2), repeatability (%Area RSD %  = 1.2–4.3%) and limit of quantitation (LOQ = 1.6–4.5 kBq). During the cross-validation similar radiochemical purity values were obtained by the novel HPLC methods and thin layer chromatography performed according to the recommendations of the Ph. Eur. monographs.

Determination of Total Radiochemical Purity of [18F]FDG and [18F]FET by High-Performance Liquid Chromatography Avoiding TLC Method