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This study examined the biological functions of the butanol extracts of green pine cones (GPCs) that had not ripened completely. The butanol extracts of GPC showed 78.22% DPPH-scavenging activity, 53.55% TEAC and 71.50% hyaluronidase (HAase) inhibition activity. They also exhibited inhibition activity against food poisoning microorganisms. The contents of total phenolic compounds and total flavonoids were 296.75 and 26.07 mg/g, respectively. Biologically active compounds were analyzed and separated using HPLC related to the DPPH-scavenging and HAase inhibition activities. Gallotannin was the primary biologically active compound with DPPH-scavenging and HAase inhibition activities in the GPC butanol extracts.

Biological properties of butanol extracts from green pine cone of Pinus densiflora