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The objective of this study was to examine the effects of glutamine on heat-shock protein beta 1 ( HSPB1 ) expression in bovine embryonic fibroblast cells during myogenesis. First, to elucidate the role of glutamine on HSPB1 expression during myogenesis, we treated with glutamine in myogenic lineage determinant (MyoD) over-expressed bovine embryonic fibroblast cells (BEFS-MyoD cells). Second, knockdown of HSPB1 using small interference RNA was performed to evaluate whether muscle development by glutamine is dependent on HSPB1 in BEFS-MyoD cells. As a result, glutamine promoted the mRNA level of HSPB1 , Myogenin , Desmin , and mTOR as well as myotube formation, and protein synthesis ( p  &lt; 0.05). The inhibition of HSPB1 expression during myogenesis has shown to repress the expression of myogenic marker genes (MyoD, Myogenin , Desmin ) ( p  &lt; 0.01), formation of myotubes and protein synthesis ( p  &lt; 0.05). According to the results, it is concluded that glutamine regulates HSPB1 expression during myogenesis.

Effect of glutamine on heat-shock protein beta 1 (HSPB1) expression during myogenic differentiation in bovine embryonic fibroblast cells