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Conjugated linoleic acid (CLA) isomers, c 9, t 11-CLA and t 10, c 12-CLA, have been proved to exhibit excellent biomedical properties for potential use in anti-cancer applications and in reducing obesity. Acer truncatum Bunge ( ATB ), which is rich in unsaturated fatty acids, including oleic acid, linoleic acid, and nervonic acid, is a new resource for edible oil. In the present study, we developed a new method for producing two CLA isomers from ATB -seed oil by fermentation using Lactobacillus plantarum CGMCC8198 ( LP 8198), a novel probiotics strain. Polymerase chain reaction results showed that there was a conserved linoleate isomerase (LIase) gene in LP 8198, and its transcription could be induced by ATB -seed oil. Analyses by gas chromatography-mass spectrometry showed that the concentration of c 9, t 11-CLA and t 10, c 12-CLA in ATB -seed oil could be increased by about 9- and 2.25-fold, respectively, after being fermented by LP 8198.

Bioconversion of conjugated linoleic acid by Lactobacillus plantarum CGMCC8198 supplemented with Acer truncatum bunge seeds oil