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Key message Expression analysis of  Larix kaempferi  mature miR171s and their primary transcripts and target gene  LaSCL6  during somatic embryogenesis revealed the transcriptional and post-transcriptional regulation of the miR171- LaSCL6  module. Somatic embryogenesis provides a useful experimental system for studying the regulatory mechanisms of plant development. The level and activity of microRNA171 (miR171) fluctuate during somatic embryogenesis in  Larix kaempferi , but the underlying mechanisms are still unclear. Here, in  L. kaempferi  we identified five members of the miR171 family, which cleave  LaSCL6  mRNA at different sites. In addition, we improved the method of measuring miRNA activity in a more direct way. Furthermore, we measured the expression patterns of mature miR171s and their primary transcripts during somatic embryogenesis in  L. kaempferi  and found that their patterns differed, indicating that the transcription of  MIR171  genes and the subsequent cleavage of their intermediate products are regulated. Taken together, our findings not only offer a means to study the regulation of miRNA activity, but also provide further insight into the regulation of  L .  kaempferi  somatic embryogenesis by miR171- LaSCL6 .

Transcriptional and post-transcriptional regulation of the miR171-LaSCL6 module during somatic embryogenesis in Larix kaempferi