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We determined whether fatigue modifies the effect of custom foot orthoses manufactured from ethyl-vinyl acetate (EVA) and expanded thermoplastic polyurethane (TPU) materials, both compared to standardized footwear (CON), on running mechanics, running economy, and perceived comfort. Eighteen well-trained, males ran on an instrumented treadmill for 6 min at the speed corresponding to their first ventilatory threshold (13.8 ± 1.1 km/h) in three footwear conditions (CON, EVA, and TPU). Immediately after completion of a repeated-sprints exercise (8 × 5 s treadmill sprints, rest = 25 s), these run tests were replicated. Running mechanics, running economy and perceived comfort were determined. Two-way repeated measures ANOVA [condition (CON, EVA, and TPU) × fatigue (fresh and fatigued)] were conducted. Flight time shortened ( P  = 0.026), peak braking ( P  = 0.016) and push-off ( P  = 0.032) forces decreased and vertical stiffness increased ( P  = 0.014) from before to after the repeated-sprint exercise, independent of footwear condition. There was a global fatigue-induced deterioration in running economy (− 1.6 ± 0.4%;  P  &lt; 0.001). There was no significant condition × fatigue [except mean loading rate ( P  = 0.046)] for the large majority of biomechanical, cardio-respiratory [except minute ventilation ( P  = 0.020) and breathing frequency ( P  = 0.019)] and perceived comfort variables. Acute intense fatigue does not modify the effect of custom foot orthoses with different resilience characteristics on running mechanics, running economy and perceived comfort.

Acute intense fatigue does not modify the effect of EVA and TPU custom foot orthoses on running mechanics, running economy and perceived comfort