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The endurance training (ET)-induced increases in peak oxygen uptake ([Formula: see text]O 2peak ) and cardiac output ([Formula: see text] peak ) during upright cycling are reversed to pre-ET levels after removing the training-induced increase in blood volume (BV). We hypothesised that ET-induced improvements in [Formula: see text]O 2peak and [Formula: see text] peak are preserved following phlebotomy of the BV gained with ET during supine but not during upright cycling. Arteriovenous O 2 difference (a-[Formula: see text]O 2 diff; [Formula: see text]O 2 /[Formula: see text]), cardiac dimensions and muscle morphology were studied to assess their role for the [Formula: see text]O 2peak improvement.

Blood volume expansion does not explain the increase in peak oxygen uptake induced by 10 weeks of endurance training