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Chargaff once said that "I saw before me in dark contours the beginning of agrammar of Biology". In linguistics, "grammar" is the set of natural languagerules, but we do not know for sure what Chargaff meant by "grammar" of Biology.Nevertheless, assuming the metaphor, Chargaff himself started a "grammar ofBiology" discovering the so called Chargaff's rules. In this work, we furtherdevelop his grammar. Using new concepts, we were able to discovery new genomicrules that seem to be invariant across a large set of organisms, and show afractal-like property, since no matter the scale, the same pattern is observed(self-similarity). We hope that these new invariant genomic rules may be usedin different contexts since short read data bias detection to genome assemblyquality assessment.

Chargaff's "Grammar of Biology": New Fractal-like Rules