Open AccessSample Preparation, Data Collection, and Preliminary Data Analysis in Biomolecular Solution X‐Ray Scattering
Author(s) -
Grishaev Alexander
Publication year - 2012
Publication title -
current protocols in protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.409
H-Index - 32
eISSN - 1934-3663
pISSN - 1934-3655
DOI - 10.1002/0471140864.ps1714s70
Subject(s) - small angle x ray scattering , scattering , data collection , sample (material) , sophistication , small angle scattering , computer science , chemistry , physics , optics , chromatography , mathematics , statistics , social science , sociology
In addition to the classic methods of structural biology—X‐ray crystallography and NMR—solution X‐ray scattering (SAXS) is playing an increasingly important role in structural investigation of biological macromolecules. However, the simultaneous ease of SAXS data collection and sophistication of its data analysis tools can present challenges to the investigator. Any sample, whether pure or contaminated, whether monodisperse or polydisperse, will yield scattering data, and it is up to the user to ensure the absence of artifacts and to choose a proper structural modeling strategy. This unit discusses experimental aspects of X‐ray solution scattering, including sample preparation and data collection, as well as the steps in data processing and preliminary analysis required to ensure the absence of artifacts. The goal is to summarize everything than can go wrong with SAXS data measurement so the user can have confidence in the data before undertaking structural modeling. Curr. Protoc. Protein Sci . 70:17.14.1‐17.14.18. © 2012 by John Wiley & Sons, Inc.