Open AccessQuantification of Glutathione in Caenorhabditis elegans
Author(s) -
Caito Samuel W.,
Aschner Michael
Publication year - 2015
Publication title -
current protocols in toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.449
H-Index - 23
eISSN - 1934-9262
pISSN - 1934-9254
DOI - 10.1002/0471140856.tx0618s64
Subject(s) - dtnb , glutathione , caenorhabditis elegans , oxidative stress , chemistry , xenobiotic , redox , biochemistry , enzyme , organic chemistry , gene
Abstract Glutathione (GSH) is the most abundant intracellular thiol with diverse functions from redox signaling, xenobiotic detoxification, and apoptosis. The quantification of GSH is an important measure for redox capacity and oxidative stress. This protocol quantifies total GSH from Caenorhabditis elegans , an emerging model organism for toxicology studies. GSH is measured using the 5,5′‐dithiobis‐(2‐nitrobenzoic acid) (DTNB) cycling method originally created for cell and tissue samples but optimized for whole worm extracts. DTNB reacts with GSH to from a 5′‐thio‐2‐nitrobenzoic acid (TNB) chromophore with maximum absorbance of 412 nm. This method is both rapid and sensitive, making it ideal for studies involving a large number of transgenic nematode strains. © 2015 by John Wiley & Sons, Inc.